[Sorbitol supply] Sorbitol content inspection and reference

Sorbitol, also known as sorbitol. It is white hygroscopic powder or crystalline powder, flakes or granules, odorless. Depending on the crystallization conditions, the melting point changes in the range of 88~102℃, and the relative density is about 1.49. Easily soluble in water (1g dissolves in about 0.45mL water), slightly soluble in ethanol and acetic acid. It has a refreshing sweetness, the sweetness is about half that of sucrose, and its caloric value is similar to that of sucrose. According to the understanding of sorbitol supply: in the food industry, sorbitol liquid with a content of 69~71% is mostly used. Sorbitol liquid is a clear, colorless, syrupy liquid with a sweet taste, neutral to litmus, and miscible with water, glycerin and propylene glycol. Can be used as nutritive sweetener, humectant, chelating agent and stabilizer.

Sorbitol content inspection and reference are as follows:

Acidity

Take 5.0g of this product, add 50ml of newly boiled and cooled water to dissolve, then add 3 drops of phenolphthalein indicator solution and sodium hydroxide 0.30ml of titrant solution (0.02mol/L) should appear light red.

Clarity and color of the solution

Take 3.0g of this product and add 20ml of water After dissolving, the solution should be clear and colorless.

Chloride

Take 1.4g of this product and check according to law (General Rules 0801 ), it must not be more concentrated (0.005%) than the control solution made from 7.0 ml of standard sodium vapor solution.

Sulfate

Take 2.0g of this product and check according to law (General Rule 0802) , compared with the control solution made from 2.0ml of standard potassium sulfate solution, it must not be more concentrated (0.01%).

Reducing sugar

Take 10.0g of this product and place it in a 400ml beaker. Add 35 ml of water to dissolve, add 50 ml of alkaline copper tartrate test solution, cover the glass dish, heat to boil within 4 to 6 minutes, continue boiling for 2 minutes, immediately add 100 ml of newly boiled water and let it cool, and use 105°C constant weight Filter through a vertical fusion glass crucible, wash the container and the precipitate in batches with 30 ml of hot water, then wash the precipitate with 10 ml of ethanol and ether in sequence, and dry to constant weight at 105°C. The weight of the obtained cuprous oxide must not exceed 67 mg.

Total sugar

Take 2.lg of this product and place it in a 250ml ground-mouth flask In, add about 40ml of hydrochloric acid solution (9-1000), heat to reflux for 4 hours, let cool, transfer the hydrochloric acid solution into a 400ml beaker, wash the container with 10ml of water and put it into the beaker, neutralize with 24% sodium hydroxide solution, and follow the instructions Under the reducing sugar item, start from “Add 50ml of alkaline copper tartrate test solution” and operate in accordance with the law. The weight of the obtained cuprous oxide must not exceed 50mg.

Related substances

Take about 0.5g of this product and place it in a 10ml measuring bottle , add water to dissolve and dilute to the mark, shake well, and use it as the test solution; accurately measure 2 ml, place it in a 100 ml measuring bottle, dilute it with water to the mark, shake well, and use it as the control solution. Take about 0.5g each of mannitol reference substance and sorbitol reference substance respectively, put them in a 10ml measuring bottle, add water to dissolve and dilute to the mark, shake well, and use it as a system suitability solution. According to the test of high performance liquid chromatography (General Chapter 0512), a strong cationic calcium type exchange column (or a chromatographic column with equivalent separation efficiency) using sulfonated cross-linked styrene divinylbenzene copolymer as filler; using water as the flow phase; the flow rate is 0.5ml per minute, the column temperature is 72-85°C, and the differential refractive index detector is used. Take 20u1 of the system suitability solution and inject it into the liquid chromatograph. The separation between the mannitol peak and the sorbitol peak should be greater than 2.0. Precisely measure 20u1 of each of the control solution and the test solution and inject them into the liquid chromatograph, and record the chromatogram to 3 times the retention time of the main component peak. If there is an impurity peak in the chromatogram of the test solution, the area of ​​a single impurity peak shall not be greater than the main peak area of ​​the control solution (2.0%), and the sum of the areas of each impurity peak shall not be greater than 1.5 times (3.0%) of the main peak area of ​​the control solution.

Weight loss on drying

Take this product and place it in a phosphorus pentoxide dryer , dry under reduced pressure at 60°C to constant weight, and the weight loss shall not exceed 1.0% (General Rule 0831).

Ignition residue

It shall not exceed 0.1% (General Rule 0841).

Heavy metals

Take 2.0g of this product and add acetate buffer (pH3.5) 2ml with an appropriate amount of water, dissolve it into 25ml, and check according to the law (General Rule 0821 First Method), the heavy metal content should not exceed 10 parts per million.

Arsenic salt

Take 1.0g of this product, add 10ml of water to dissolve it, Add 5 ml of dilute sulfuric acid and 0.5 ml of potassium bromide bromine test solution, place it on a water bath and heat for 20 minutes to maintain a slight excess of bromine (if necessary, add potassium bromide bromine test solution dropwise), and replenish evaporated water at any time. Let cool, add 5ml of hydrochloric acid and an appropriate amount of water to make 28ml. Check according to the law (General Rules 0822 Law 1), it should meet the regulations (0.0002%).